Vaporized hydrogen peroxide, also known as vaporized hydrogen peroxide, abbreviated as VHP, is a technology that utilizes the advantage of hydrogen peroxide having a stronger ability to kill bacterial spores in a gaseous state than in a liquid state at room temperature, achieving complete sterilization requirements. The VHP transfer window we usually refer to is the vaporized hydrogen peroxide transfer window, which is an integrated transfer window that directly installs the device for vaporized hydrogen peroxide generator inside the transfer window.

By high-temperature flash evaporation, hydrogen peroxide liquid is vaporized into hydrogen peroxide gas, which is then sprayed into the sterilization space through high-speed airflow. When the high-temperature saturated hydrogen peroxide vapor comes into contact with the colder surface of the disinfected item, it forms invisible micro condensation, which attacks pathogenic microorganisms (destroying cell membranes, lipids, proteins, and DNA) through the release of strong oxidative free radicals (hydroxyl groups). Free radicals can achieve a rapid killing effect of log6 on microorganisms. After sterilization, the vaporized hydrogen peroxide sterilization equipment will automatically decompose hydrogen peroxide molecules in the environment into water vapor and oxygen. When the concentration of hydrogen peroxide in the space drops below 1ppm, sterilization is complete. The VHP transfer window adopts dry sterilization technology, which kills bacteria by reducing the humidity of the space and increasing the concentration of hydrogen peroxide. The entire operation process requires dehumidification of the sterilization space, and the humidity is generally required to be controlled below 30%.

Sterilization process
Cavity pressure maintenance
PART 01
In the early stage of each sterilization, the chamber is pressurized and then subjected to a pressure holding process until the pressure holding is confirmed to be correct before proceeding to the next step. This step is to ensure the airtightness of the hydrogen peroxide sterilization transfer window, prevent hydrogen peroxide leakage, ensure the safety of on-site operators, and prevent environmental damage.
Self purification of cavity
PART 02
After pressure confirmation, use the inlet fan and exhaust fan to perform a gas replacement operation on the chamber for about 2 minutes, completely exhausting the air inside the chamber to achieve self purification and prepare for the next step of heating and dehumidification.
Chamber heating and dehumidification
PART 03
After self-cleaning is completed, use a rotary dehumidifier to heat up and dehumidify the chamber. This step is to increase the temperature inside the chamber and reduce the humidity inside the chamber, providing ****** environmental conditions for the sterilization of hydrogen peroxide. Excessive air humidity or low ambient temperature can reduce the effectiveness of disinfection and sterilization. Therefore, before carrying out disinfection and sterilization work, it is necessary to clean and dry the disinfection and sterilization space. By controlling the rotary dehumidifier, all monitoring values during the heating and dehumidification process can be effectively controlled and the program logic judgment can be made.
Sterilization enhancement
PART 04
After the heating and dehumidification are completed, use a hydrogen peroxide generator to inject hydrogen peroxide gas into the interior of the chamber, accelerate the concentration of hydrogen peroxide in the chamber in a short period of time, and quickly reach the sterilization lower limit concentration value of ≥ 300ppm.
Sterilization maintenance
PART 05
After reaching the sterilization lower limit concentration value, maintain the temperature and humidity of the chamber within a controllable range and keep the concentration ≥ 300ppm for a certain period of time. This process adopts an internal circulation method, where the hydrogen peroxide gas is replaced inside the chamber through the circulation of the dehumidifier, inlet fan, and exhaust fan. This step is to ensure that hydrogen peroxide can fully contact and kill all microorganisms inside the chamber.
Degradation and removal of residues
PART 06
After sterilization is completed, the hydrogen peroxide gas inside the chamber is replaced by a residual exhaust fan to quickly exhaust the concentration of hydrogen peroxide inside the chamber to within 1ppm, ensuring that the operator will not be harmed by hydrogen peroxide when opening the transfer window. The equipment is equipped with an integrated catalytic filtration device to ensure that the discharge interlayer can also operate without causing risks and damage to the interlayer construction and environment.
The hydrogen peroxide sterilization transfer window mainly includes: door sealing system, high-efficiency filtration system, peristaltic pump system, inflation and pressure holding system, temperature and humidity control system, loading mode, etc.
The hydrogen peroxide sterilization transfer window uses a 35% high concentration hydrogen peroxide solution to flash evaporate into gas and transport it to the interior of the transfer window chamber for sterilization. The entire process is monitored in real-time, and through process development and PID process adjustment (such as injection volume, temperature and humidity control, air volume, etc.), the surface sterilization of the transferred material is effectively ensured, while no hydrogen peroxide condensate remains on the surface of the material, making its sterilization surface always dry. It comes with an efficient filter for self-cleaning function, a dual door design, and uses JHAO's inflatable sealing technology certified by a third party to seal and isolate the chamber, and achieve double door interlocking to prevent cross infection.
Hydrogen peroxide, as an important environmentally friendly disinfectant, has been widely used in recent years. Especially with the development and launch of automated sterilization technology using vaporized hydrogen peroxide and hydrogen peroxide plasma sterilizers, hydrogen peroxide disinfection has experienced explosive growth.
Vaporized hydrogen peroxide can be used for space disinfection, such as in biosafety laboratories, GMP workshops, hospital wards, experimental animal centers, and various biosafety equipment (such as isolators, incubators, transfer windows, biosafety cabinets, high-efficiency filtration units, and various ventilation ducts). It is a reliable disinfection and sterilization method with broad development prospects. With the further integration of automation control technology and traditional disinfection methods, more standardized and safer hydrogen peroxide disinfection technology will be used in various disinfection fields and play important social and economic benefits.
Hydrogen peroxide plasma sterilization requires vacuuming of the chamber, which is relatively limited in space and more suitable for sterilization of medical devices, instruments, and items that are not resistant to moisture and high temperatures. The price is also relatively expensive.

上海杰昊生物技術(shù)有限公司位于上海市奉賢區(qū)，專注生物安全設備的研發(fā)和生產(chǎn)十余載，在潔凈行業(yè)深耕20余年，為客戶提供高等級生物安全實驗室整體氣密性解決方案。公司產(chǎn)品主要有：生物安全氣密門，生物安全傳遞窗，生物安全渡槽，化學淋浴，強制淋浴，霧淋室，VHP傳遞窗，過氧化氫空間滅菌設備，不銹鋼密閉房，稱量罩等產(chǎn)品。